Review



mouse spleen lymphocyte isolation solution kit  (Beijing Solarbio Science)


Bioz Verified Symbol Beijing Solarbio Science is a verified supplier
Bioz Manufacturer Symbol Beijing Solarbio Science manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Beijing Solarbio Science mouse spleen lymphocyte isolation solution kit
    Mouse Spleen Lymphocyte Isolation Solution Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+spleen+lymphocyte+isolation+solution+kit/Mouse+Spleen+Lymphocyte+Isolation+Solution+Kit/pmc13045921-159-12-18
    Average 96 stars, based on 138 article reviews
    mouse spleen lymphocyte isolation solution kit - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Oxymatrine attenuates the type 1 diabetes mellitus via negative regulation of the follicular helper T cells.
    Article Snippet: Type 1 diabetes mellitus (T1DM) is an autoimmune disorder in which autoantibodies cause the immune system to attack and destroy pancreatic β-cells, leading to insufficient insulin production and impaired blood glucose control.. T follicular helper (Tfh) cells are recognized as a group of CD4 T cells that help B cells to produce highaffinity antibodies.. Our previous research found that oxymatrine (OMT) exhibits excellent immunomodulatory properties on Tfh cells in autoimmune diseases.

    Article Title: An Engineered Nano‐Vesicle Adjuvant Platform (ENAP) for Cytokine Delivery Enables a Novel Antigen‐Coordinated Vaccine Against Helicobacter pylori
    Article Snippet: .. Two weeks post H. pylori challenge, spleen lymphocytes were isolated using a mouse spleen lymphocyte isolation solution kit (Solarbio), single‐cell suspensions were then plated in 12‐well plates and stimulated with 6 μg/mL UreB protein or OMPs (endotoxin <100 EU/mg) in RPMI‐1640 medium supplemented with 100 IU/mL penicillin‐streptomycin and 10% FBS (Gibco), followed by incubation at 37°C under 5% CO 2 for 24 h. During the final 6 h of incubation, protein transport inhibitors brefeldin A (2 μL/mL; BD Biosciences) and monensin (1.4 μL/mL; BD Biosciences) were added to the cells. .. After centrifugation and supernatant removal, 2 μL of Fc receptor blocker (BioLegend) and 5 μL of pre‐mixed True‐Stain monocyte blocker (BioLegend) were added to the cell pellet and incubated for 10 min.

    Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis
    Article Snippet: .. Subsequently, spleen lymphocytes were extracted and purified by using the mouse spleen lymphocyte isolation solution kit (Solarbio, Beijing, China) according to the manufacturers’ instructions. .. After purification, naive CD8 + T cells were isolated using the Mouse Naive CD8 + T cell Isolation Kit ( #19858 , EasySep, Stemcell, Canada).

    Article Title: Novel plant-derived exosome-like nanovesicles from Catharanthus roseus: preparation, characterization, and immunostimulatory effect via TNF-α/NF-κB/PU.1 axis.
    Article Snippet: RAW264.7 cells were plated into a 96-well plate at 1 × 106 cells/well, and then were treated with CLDENs, CrDDENs, CrCDENs or LPS 24 h. After another 24 h of incubation, the cell culture supernatant was collected and the concentration of TNF-α, IL-6, IL-1β, IL-10 were assessed using ELISA kits (ExCell, Shanghai, CHN). .. Mouse spleen lymphocytes were extracted according to the instructions of the Mouse Spleen Lymphocyte Isolation Solution Kit (Solarbio, Beijing, CHN). .. Lymphocytes were inoculated in 96-well plates at 5.0 × 106 cells/well and incubated with CLDENs (30–240 μg/ml) for 24 h. Then, the cell culture supernatant was collected and the concentration of IL-2 were assessed using ELISA kits (ExCell, Shanghai, CHN).

    Article Title: An Engineered Nano-Vesicle Adjuvant Platform (ENAP) for Cytokine Delivery Enables a Novel Antigen-Coordinated Vaccine Against Helicobacter pylori.
    Article Snippet: .. Two weeks post H. pylori challenge, spleen lymphocytes were isolated using a mouse spleen lymphocyte isolation solution kit (Solarbio), single-cell suspensions were then plated in 12- well plates and stimulated with 6 μg/mL UreB protein or OMPs (endotoxin < 100 EU/mg) in RPMI-1640 medium supplemented with 100 IU/mL penicillin-streptomycin and 10% FBS (Gibco), followed by incubation at 37◦C under 5% CO2 for 24 h. During the final 6 h of incubation, protein transport inhibitors brefeldin A (2 μL/mL; BD Biosciences) and monensin (1.4 μL/mL; BD Biosciences) were added to the cells. .. After centrifugation and supernatant removal, 2 μL of Fc receptor blocker (BioLegend) and 5 μL of pre-mixed True-Stain monocyte blocker (BioLegend) were added to the cell pellet and incubated for 10 min.

    Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis.
    Article Snippet: .. Subsequently, spleen lymphocytes were extracted and purified by using the mouse spleen lymphocyte isolation solution kit (Solarbio, Beijing, China) according to the manufacturers’ instructions. .. After purification, naive CD8+ T cells were isolated using theMouse Naive CD8+ T cell Isolation Kit (#19858, EasySep, Stemcell, Canada).

    Article Title: Potentiating immunotherapy in “immune-cold” solid tumors through orchestrating T cell immunity via tumor-specific genetic engineering
    Article Snippet: Mouse Peripheral Blood Lymphocyte Isolation Solution Kit , Solarbio , P8620. .. Mouse Spleen Lymphocyte Isolation Solution Kit , Solarbio , P8860. .. HisPurTM Ni-NTA Spin Purification Kit , Thermo , 88229.

    Single Cell:

    Article Title: An Engineered Nano‐Vesicle Adjuvant Platform (ENAP) for Cytokine Delivery Enables a Novel Antigen‐Coordinated Vaccine Against Helicobacter pylori
    Article Snippet: .. Two weeks post H. pylori challenge, spleen lymphocytes were isolated using a mouse spleen lymphocyte isolation solution kit (Solarbio), single‐cell suspensions were then plated in 12‐well plates and stimulated with 6 μg/mL UreB protein or OMPs (endotoxin <100 EU/mg) in RPMI‐1640 medium supplemented with 100 IU/mL penicillin‐streptomycin and 10% FBS (Gibco), followed by incubation at 37°C under 5% CO 2 for 24 h. During the final 6 h of incubation, protein transport inhibitors brefeldin A (2 μL/mL; BD Biosciences) and monensin (1.4 μL/mL; BD Biosciences) were added to the cells. .. After centrifugation and supernatant removal, 2 μL of Fc receptor blocker (BioLegend) and 5 μL of pre‐mixed True‐Stain monocyte blocker (BioLegend) were added to the cell pellet and incubated for 10 min.

    Article Title: An Engineered Nano-Vesicle Adjuvant Platform (ENAP) for Cytokine Delivery Enables a Novel Antigen-Coordinated Vaccine Against Helicobacter pylori.
    Article Snippet: .. Two weeks post H. pylori challenge, spleen lymphocytes were isolated using a mouse spleen lymphocyte isolation solution kit (Solarbio), single-cell suspensions were then plated in 12- well plates and stimulated with 6 μg/mL UreB protein or OMPs (endotoxin < 100 EU/mg) in RPMI-1640 medium supplemented with 100 IU/mL penicillin-streptomycin and 10% FBS (Gibco), followed by incubation at 37◦C under 5% CO2 for 24 h. During the final 6 h of incubation, protein transport inhibitors brefeldin A (2 μL/mL; BD Biosciences) and monensin (1.4 μL/mL; BD Biosciences) were added to the cells. .. After centrifugation and supernatant removal, 2 μL of Fc receptor blocker (BioLegend) and 5 μL of pre-mixed True-Stain monocyte blocker (BioLegend) were added to the cell pellet and incubated for 10 min.

    Incubation:

    Article Title: An Engineered Nano‐Vesicle Adjuvant Platform (ENAP) for Cytokine Delivery Enables a Novel Antigen‐Coordinated Vaccine Against Helicobacter pylori
    Article Snippet: .. Two weeks post H. pylori challenge, spleen lymphocytes were isolated using a mouse spleen lymphocyte isolation solution kit (Solarbio), single‐cell suspensions were then plated in 12‐well plates and stimulated with 6 μg/mL UreB protein or OMPs (endotoxin <100 EU/mg) in RPMI‐1640 medium supplemented with 100 IU/mL penicillin‐streptomycin and 10% FBS (Gibco), followed by incubation at 37°C under 5% CO 2 for 24 h. During the final 6 h of incubation, protein transport inhibitors brefeldin A (2 μL/mL; BD Biosciences) and monensin (1.4 μL/mL; BD Biosciences) were added to the cells. .. After centrifugation and supernatant removal, 2 μL of Fc receptor blocker (BioLegend) and 5 μL of pre‐mixed True‐Stain monocyte blocker (BioLegend) were added to the cell pellet and incubated for 10 min.

    Article Title: An Engineered Nano-Vesicle Adjuvant Platform (ENAP) for Cytokine Delivery Enables a Novel Antigen-Coordinated Vaccine Against Helicobacter pylori.
    Article Snippet: .. Two weeks post H. pylori challenge, spleen lymphocytes were isolated using a mouse spleen lymphocyte isolation solution kit (Solarbio), single-cell suspensions were then plated in 12- well plates and stimulated with 6 μg/mL UreB protein or OMPs (endotoxin < 100 EU/mg) in RPMI-1640 medium supplemented with 100 IU/mL penicillin-streptomycin and 10% FBS (Gibco), followed by incubation at 37◦C under 5% CO2 for 24 h. During the final 6 h of incubation, protein transport inhibitors brefeldin A (2 μL/mL; BD Biosciences) and monensin (1.4 μL/mL; BD Biosciences) were added to the cells. .. After centrifugation and supernatant removal, 2 μL of Fc receptor blocker (BioLegend) and 5 μL of pre-mixed True-Stain monocyte blocker (BioLegend) were added to the cell pellet and incubated for 10 min.

    Purification:

    Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis
    Article Snippet: .. Subsequently, spleen lymphocytes were extracted and purified by using the mouse spleen lymphocyte isolation solution kit (Solarbio, Beijing, China) according to the manufacturers’ instructions. .. After purification, naive CD8 + T cells were isolated using the Mouse Naive CD8 + T cell Isolation Kit ( #19858 , EasySep, Stemcell, Canada).

    Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis.
    Article Snippet: .. Subsequently, spleen lymphocytes were extracted and purified by using the mouse spleen lymphocyte isolation solution kit (Solarbio, Beijing, China) according to the manufacturers’ instructions. .. After purification, naive CD8+ T cells were isolated using theMouse Naive CD8+ T cell Isolation Kit (#19858, EasySep, Stemcell, Canada).



    Similar Products

    mouse spleen lymphocyte isolation solution kit  (Beijing Solarbio Science)
    96
    Beijing Solarbio Science mouse spleen lymphocyte isolation solution kit
    Mouse Spleen Lymphocyte Isolation Solution Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+spleen+lymphocyte+isolation+solution+kit/Mouse+Spleen+Lymphocyte+Isolation+Solution+Kit/pmc13045921-159-12-18
    Average 96 stars, based on 1 article reviews
    mouse spleen lymphocyte isolation solution kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    mouse spleen lymphocyte isolation kit  (Beijing Solarbio Science)
    96
    Beijing Solarbio Science mouse spleen lymphocyte isolation kit
    Mouse Spleen Lymphocyte Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+spleen+lymphocyte+isolation+solution+kit/Mouse+Spleen+Lymphocyte+Isolation+Solution+Kit/pm41897411-82-1-9
    Average 96 stars, based on 1 article reviews
    mouse spleen lymphocyte isolation kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    mouse splenic lymphocyte isolation kit  (Beijing Solarbio Science)
    96
    Beijing Solarbio Science mouse splenic lymphocyte isolation kit
    Mouse Splenic Lymphocyte Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+spleen+lymphocyte+isolation+solution+kit/Mouse+Spleen+Lymphocyte+Isolation+Solution+Kit/pmc12882968-50-24-30
    Average 96 stars, based on 1 article reviews
    mouse splenic lymphocyte isolation kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    mouse spleen lymphocyte separation kit  (Beijing Solarbio Science)
    96
    Beijing Solarbio Science mouse spleen lymphocyte separation kit
    Mouse Spleen Lymphocyte Separation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+spleen+lymphocyte+isolation+solution+kit/Mouse+Spleen+Lymphocyte+Isolation+Solution+Kit/pm41616890-87-15-23
    Average 96 stars, based on 1 article reviews
    mouse spleen lymphocyte separation kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    mouse spleen  (Beijing Solarbio Science)
    96
    Beijing Solarbio Science mouse spleen
    Mouse Spleen, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+spleen+lymphocyte+isolation+solution+kit/Mouse+Spleen+Lymphocyte+Isolation+Solution+Kit/10__1002_slash_inmd__70089-199-27-29
    Average 96 stars, based on 1 article reviews
    mouse spleen - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results